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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Monoclonal anti Calbindin D-28k 200ul
品牌:Swant
貨號(hào):300
規(guī)格:300
貨期:swant
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瑞士Swant?公司,瑞士一家生物科技公司,產(chǎn)品包括用于鈣結(jié)合蛋白(D-28k),鈣調(diào)蛋白,離子通道蛋白,神經(jīng)遞質(zhì)的抗體和抗原,用于神經(jīng)元或神經(jīng)膠質(zhì)細(xì)胞及癌細(xì)胞的染色產(chǎn)品和用于免疫組合的單抗和多抗。多年來專注于鈣結(jié)合蛋白產(chǎn)品的研究,其產(chǎn)品雖種類雖然不多,但是卻都經(jīng)典熱銷,并且經(jīng)常出現(xiàn)在高分的文章中。為國(guó)內(nèi)外眾多相關(guān)領(lǐng)域科學(xué)家所熱衷。

商品詳情 參考文獻(xiàn) 相關(guān)資料

產(chǎn)品介紹

品名

Monoclonal anti Calbindin D-28k 

貨號(hào)

300

規(guī)格

200ul / 500ul  / 1ml

 克隆性

monoclonal

共軛

nonconjugated

反應(yīng)性

thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque

應(yīng)用

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

宿主動(dòng)物

Mouse monoclonal, IgG1

抗體形式

Conc. supernat. lyophil.

產(chǎn)品形式

Lyophilized concentrated supernatant (no preservatives). 

復(fù)溶

with 200 μl / 500 μl or 1 ml bidistilled water

產(chǎn)品描述

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

稀釋

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

We recommend that the optimal dilutions be determined by titration experiments.

儲(chǔ)存

For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

參考文獻(xiàn)

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

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