Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM205 at 1 ug/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.
RM205 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody (clone RM205) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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