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Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140

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Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140

品牌：RevMAb

貨號(hào)：31-1046-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM140]
Product Description	Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4)
Catalog No.	31-1046-00
Clone Name	RM140
Product Data Sheet	Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140
Specificity	RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3
Immunogen	A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)
Application	Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.2 ug/mL – 1 ug/mL; ChIP: 2 ug/mL- 10 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM140 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM140 at 0.5 ug/mL, showed a band of histone H3 monomethylated at Lysine4 (K4me1) in HeLa cells.
	ChIP performed on HeLa cells using H3K4me1 antibody (RM140, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Monomethyl-Histone H3(Lys4) Rabbit mAb RM140 (red). Actin filaments have been labeled with fluorescein phalloidin (green).