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Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody, Clone RM201; H4K8ac, Histone H4 (K8 acetylation); ChIP Validated

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Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody, Clone RM201; H4K8ac, Histone H4 (K8 acetylation); ChIP Validated

品牌：RevMAb

貨號(hào)：31-1082-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody [RM201]
Product Description	Rabbit monoclonal to Acetylated Histone H4 Lysine 8 (K8ac), H4K8ac, Histone H4 (K8 acetylation)
Catalog No.	31-1082-00
Clone Name	RM201
Product Data Sheet	Anti-Acetyl-Histone H4 Lys8 Rabbit Monoclonal Antibody Data Sheet RM201
Specificity	RM201 reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8).
Application	Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC)
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 1 ug/mL; Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords
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Product Images
	RM201 specifically reacts to Histone H4 acetylatedat Lysine 8 (K8ac). No cross reactivity with unmodifiedLysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
	Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using RM201 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
	Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody (clone RM201) (red). Actin filaments have been labeled with fluorescein phalloidin (green).