Anti-Dimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM141
Specificity
RM141 reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in histone H3.
Immunogen
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys36).
Application
Western Blot (WB)
ELISA
Multiplex
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
RM141 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non -modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 (K36me2) in HeLa cells.