Anti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody Data Sheet RM168
Specificity
RM168 reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18, monomethylated Lysine 18 (K18me1), or other methylations in histone H3.
Immunogen
A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys18).
Application
Western Blot (WB)
ELISA
Multiplex
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
A Peptide dotblot shows RM168 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity. with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2),. or dimethylated Lysine 36 (K36me2).
RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.