Anti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM157
Specificity
RM157 reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with monomethylated Lysine 79 (K79me1), dimethylated Lysine 79 (K79me3), or other methylations in histone H3.
Immunogen
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys79).
Application
Western Blot (WB)
ELISA
Multiplex
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
A Peptide dotblot shows RM157 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity. with nonmodified Lysine 79 (H3K79Ctrl), monomethylated. Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).
RM157 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells.