Anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody Data Sheet RM235
Specificity
RM235 reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).
Application
Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non- modified Lysine 20 or other acetylated Lysines in histone H2B.
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM235 at 0.5 ug/mL.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (clone RM235) (red). Actin filaments have been labeled with fluorescein phalloidin (green).