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產(chǎn)品介紹
品名	Rabbit anti-Tescalcin
貨號	T876
規(guī)格	200ul
克隆性	polyclonal
共軛	rabbit
反應性	nonconjugated
宿主動物	Rabbit
抗體形式	Antiserum lyophil.
產(chǎn)品形式	Lyophilized whole serum (no preservatives)
復溶	with 200 μl of bi-distilled water.
產(chǎn)品描述	The antiserum against Tescalcin is produced in rabbits by immunization with recombinant mouse Tescalcin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde fixed brains and b) by immunoblots (Girard et al, 2015).
產(chǎn)品用途	Tescalcin is a novel calcineurin B-like protein that binds a single Ca2+ ion. It occurs mainly in the heart; stomach and brain (see also the Allen brain atlas (http://www.brainmap.org/).
稀釋	Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections.  Immunoblots: 1:1'000 - 1:2'000. For immunohistochemistry and immunoblots the titre was determined by using the avidin-biotin method.  We recommend that the optimal dilutions be determined by titration experiments.
儲存	After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
參考文獻	Girard F, Venail J, Schwaller B, Celio MR. (2015) The EF-hand Ca(2+)-binding protein super-family: a genome-wide analysis of gene expression patterns in the a dult mouse brain.Neuroscience;294:116-55.