G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Recombinant Rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues.
Source: E.coli
Synonyms: Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFβ, pluripoietin
AA Sequence: MKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
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