T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Application
? Cloning of restriction fragments ? Attaching double-stranded oligonucleotide linkers or adaptors to DNA ? Site-directed mutagenesis ? Nick repair of duplex DNA, RNA or DNA-RNA hybrids ? Self-circularization of linear DNA
Form
Enzyme supplied with 5X Reaction Buffer
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Concentration
1 U/μl
Storage
Store all components at -20°C.
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.