Anti-HIF-1-alpha Rabbit Monoclonal Antibody Data Sheet RM242
Specificity
RM242 reacts to human HIF-1-alpha (Hypoxia-inducible factor 1-alpha).
Immunogen
A peptide corresponding to Hypoxia-inducible factor 1-alpha
Application
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity
Human
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size
100 uL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Clone Type
Monoclonal
Isotype
Rabbit IgG
Usage
Immunohistochemistry (IHC): 1:500-1:1000 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242 at a 1:1000 dilution.
Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt(II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expression was induced by CoCl2 in Jurkat cells.